Method verification study for detection of Cryptosporidium spp. by realtime PCR method
نویسندگان
چکیده
منابع مشابه
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متن کاملMethod detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil.
We determined and compared the method detection limits (MDLalpha) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDLalpha and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil studies of oocyst transport.
متن کاملDetection and differentiation of Cryptosporidium spp. in human clinical samples by use of real-time PCR.
Real-time PCR has the potential to streamline detection and identification of Cryptosporidium spp. in human clinical samples. In the present article, we report the first such assay to allow not only detection and differentiation of the most common human pathogens, Cryptosporidium hominis and Cryptosporidium parvum, but also simultaneous amplification of a region of the small subunit (SSU) rRNA ...
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ژورنال
عنوان ژورنال: Türk Mikrobiyoloji Cemiyeti Dergisi
سال: 2019
ISSN: 0258-2171
DOI: 10.5222/tmcd.2019.162